01.20.10 Lecture 4 Cellular Building Blocks: Lipids and Membranes .

Uploaded on:
01.20.10 Lecture 4 Cellular Building Blocks: Lipids and Membranes. Membranes form many compartments in the cell. Biological membranes are composed of a lipid bilayer. Membrane lipids are amphipathic molecules. Membrane lipids are amphipathic
Slide 1

01.20.10 Lecture 4 Cellular Building Blocks: Lipids and Membranes

Slide 2

Membranes structure numerous compartments in the phone

Slide 3

Biological layers are made out of a lipid bilayer

Slide 4

Membrane lipids are amphipathic atoms Membrane lipids are amphipathic Hydrophilic heads (polar) structure hydrogen securities with water Hydrophobic tails (non-polar) are prohibited by water particles

Slide 5

Three classes of film lipids

Slide 6

Membrane lipids Phosphatidylcholine is the most rich phospholipid in cell layers

Slide 7

Packing courses of action of lipid particles in a watery situation Cone-molded lipid particles for micelles, chamber molded lipids structure bilayers

Slide 8

Phospholipid bilayers suddenly near structure a fixed compartment

Slide 9

The layer bilayer is a liquid Lateral dispersion happens quickly inside the plane of the film Individual phospholipids may pivot pivotally Flip-floundering from one side to the next is extremely uncommon as it is enthusiastically unfavorable

Slide 10

The film bilayer is a liquid: FRAP A fluorescent test is utilized to mark layer proteins The test is devastated in a little district utilizing exceptional laser light Fluorescence microscopy is utilized to watch conduct of the unbleached test

Slide 11

The film bilayer is a liquid: single particle imaging Fluorescence microscopy of single GFP-named layer proteins Diffusive development inside the plane of the lipid bilayer

Slide 12

The layer bilayer is a liquid: laser tweezers Beads caught in a laser might be utilized to apply pulling strengths on layers

Slide 13

The sythesis of a film directs the level of its ease Membrane lipids with greasy acyl side chains that are immersed (no twofold securities) pack firmly in the film and make it less liquid Lipids that are unsaturated (1, 2, or 3 twofold securities) pack freely and make it more liquid

Slide 14

The creation of a film controls the level of its ease The nearness of cholesterol in the film solidifies the bilayer making it more unbending

Slide 15

Cellular layers are unbalanced All lipids are blended on the cytosolic surface of the ER Lipids in the external flyers are transported there by flippases Continuity between organelle lumen & extracellular space

Slide 16

Membrane Proteins make ~50% out of the layer ~1 protein:50 lipid particles Membrane proteins perform numerous capacities

Slide 17

Membrane proteins partner with the bilayer in various ways Transmembrane proteins traverse the bilayer Peripheral layer proteins partner with one side

Slide 18

Transmembrane proteins normally traverse the bilayer utilizing alpha-helices

Slide 19

Some layer proteins use beta-sheets to cross the bilayer Beta-sheets masterminded in this tube shaped adaptation are known as a "beta-barell" Hydrophilic amino corrosive buildups face towards the pore, hydrophobics face the bilayer

Slide 20

The cytoplasmic side of the layer is known as the phone cortex Meshwork of transmembrane proteins and fibers (spectrin) Mechanical backing for the film and cell shape

Slide 21

The extracellular surface of the film is covered with sugar

Slide 22

Extracellular glycoproteins play out various capacities Carbohydrate layer shields cells from synthetic and mechanical harm Different cell sorts present diverse mixes of glycoproteins and proteoglycan on their surface - sub-atomic mark Information in the starch layer helps in cell-cell acknowledgment and correspondence

Slide 23

Cells use distinctive instruments to confine layer protein developments By tying to components within the phone (cortex) By tying to components outside of the phone By connecting with proteins on the surface of another phone By dissemination hindrances built up inside enraptured cells

Slide 24

Epithelial cell extremity

View more...