Address 14.


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Address 14 LC-MS Ionization GC-MS GC MS PC LC-MS LC MS PC LC? MS Investigation MS Gas stage Particles Dense Stage Ionization Electrospray Ionization (ESI) Air Weight Compound Ionization (APCI) Air Weight Photoionization (APPI)
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Slide 1

Address 14 LC-MS Ionization

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GC-MS GC MS Computer

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LC-MS LC MS Computer

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LC? MS Analysis MS Gas stage Ions

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Condensed Phase Ionization Electrospray Ionization (ESI) Atmospheric Pressure Chemical Ionization (APCI) Atmospheric Pressure Photoionization (APPI) Matrix Assisted Laser Desorption/Ionization (MALDI) Desorption Electrospray Ionization (DESI) Direct Analysis in Real Time (DART) Liquid stage (LC) Solid stage

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PCBs Production began 1920s Env Detection 1968 Difference? PFOS Production began 1930s Env Detection 2001

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MS Analysis GC-MS and LC-MS ionization are altogether different GC – Producing particles from gas stage analytes Producing gas stage mixes from charged analytes LC – Necessary for certain imperative analytes: Biological atoms: Proteins Amino acids/nucleic acids sugars Strong acids without the capacity to ingestion UV-vis

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Problem solved… Electrospray Ionization (ESI) John Fenn

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LC Ionization Chamber Capillary Gas Heater MS Nebulized Spray Sample Cone Electrospray Ionization (ESI) ESI Key Features: - Capillary (LC) 90 o to Cone (MS) - Atmospheric weight particle source - Nebulized course through slender - Heat connected to vanish dissolvable - Voltage distinction connected in the middle of narrow and cone + - Detect (+) particles  Cap (+), Cone (- ) - Detect (- ) particles  Cap (- ), Cone (+) - 10 - 6 torr What happens in the bead? 760 torr

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Electrospray Ionization (ESI) + Interested in Positive Ions - Apply (+) Voltage to slender - (+) particles repulsed from fine - (- ) particles pulled in to slim - Results in beads improved in (+) particles + P. Kebarle, J. Mass Spectrom. 2000 , 35 , 804–817.

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Electrospray Ionization (ESI) Summary Ions are framed in consolidated stage In portable stage beads Ions move into the gas stage by two instruments: Partition into gas stage because of columbic aversion in drop Solvent in drop vanishes abandoning particle Works well for intensifies that can be charged in the dense stage Strong or frail Acids and Bases

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LC Ionization Chamber Capillary Gas Heater Nebulized Spray Sample Cone Atmospheric Pressure Chemical Ionization (APCI) APCI Key Features: - Capillary (LC) 90 o to Cone (MS) - Atmospheric weight particle source - Nebulized move through hairlike - Heat connected to dissipate dissolvable - No voltage connected to slim - drops are not charged - Voltage still connected to cone - Detect (- ) particles  Cone (+) - Detect (+) particles  Cone (- ) MS - Heat vanishes dissolvable, analytes allotment into the gas-stage uncharged 10 - 6 torr Corona release needle 760 torr - Need to make particles!

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Atmospheric Pressure Chemical Ionization (APCI) Summary First step is dissolvable vanishing Compounds in gas-stage as neutrals Ions created in the gas-stage Corona release ionizes transporter gas and portable stage Acid/base responses in the gas stage with versatile stage Works well for aggravates that are protic however unbiased at a pH somewhere around 2 and 9 Weak Acids/Bases

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LC Ionization Chamber Capillary Gas Heater Nebulized Spray Sample Cone Atmospheric Pressure Photoionization (APPI) APPI Key Features (same as APCI): - Capillary (LC) 90 o to Cone (MS) - Atmospheric weight particle source - Nebulized move through slim - Heat connected to dissipate dissolvable - No voltage connected to fine - beads are not charged - Voltage still connected to cone - Detect (- ) particles  Cone (+) - Detect (+) particles  Cone (- ) MS UV light - Heat vanishes dissolvable, analytes allotment into the gas-stage uncharged 10 - 6 torr 760 torr - Need to make particles!

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Atmospheric Pressure Photoionization (APPI) Summary First step is dissolvable vanishing Compounds in gas-stage as neutrals Ions delivered in the gas-stage UV light ionizes compound by photon retention or Charge trade with dopant or Dopant ionizes versatile stage, trailed by; Acid/base responses in the gas stage with portable stage Works well for exacerbates that are retain UV radiation or are protic yet nonpartisan at a pH somewhere around 2 and 9 Weak Acids/Bases Nonpolar aggravates that are UV dynamic

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Summary LC-MS Ionization m/z (MW) www.agilent.com

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+ - + Matrix-Assisted Laser Desorption/Ionization (MALDI) MS MALDI Key Features: Vacuum - Analyte stored on surface with overabundance lattice compound - Shine laser at first glance - h n aborbed by network particles - Matrix atoms exchange vitality to analyte particles - Results in desorption/ionization of both framework and analyte Laser shaft Analyte particle Matrix particle Adapted from Dass, C. Essentials of Contemporary Mass Spectrometry , Wiley-Interscience, 2007.

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Matrix-Assisted Laser Desorption/Ionization (MALDI) Common dopants: Analytes of interest: Proteins Polymers High MW, ionization unrealistic utilizing ESI, APCI, APPI High MW isolates these mixes from the foundation sign of the lattice

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Desorption Electrospray Ionization (DESI) Z. Tak á ts et al. Science 2004 , 306 , 471-473.

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Direct Analysis in Real Time (D

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