BC fundamental - from huge explosion .


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How to Separate These Objects . 1. 3. 2. 6. 8. 5. 4. 9. 7. 10. 11. 12. 1. 3. 2. cotton. 8. 4. wood. 5. 6. 8. 4. 7. 5. stone. 9. 10. 11. 12. Shape Size Density. wood stone cotton wood wood cotton stone wood stone cotton stone cotton. Shape. 4. 6. Size.
Transcripts
Slide 1

The most effective method to Separate These Objects 1 3 2 6 8 5 4 9 7 10 11 12 1 3 2 cotton 8 4 wood 5 6 8 4 7 5 stone 9 10 11 12 Shape Size Density wood stone cotton wood cotton stone wood stone cotton stone cotton Shape 4 6 Size 8 5 Density 7 Different moving pace Different sedimentation Sieving diverse sizes Juang RH (2004) BCbasics

Slide 2

Basic Principles of Protein Purification Cell Macromolecule Organelle Homogenization Small atom Cell Debris Amino corrosive, Sugar, Nucleotides, and so forth Protein Nucleic corrosive Carbohydrate (Lipid) Ammonium sulfate fractionation Si ze Charge Polarity Affinity Ion trade, Chromatofocusing , Disk-PAGE, Isoelectric centering Reverse stage chromatography, HIC , Salting-out Gel filtration , SDS-PAGE , Ultrafiltration Affinity chromatography , Hydroxyapatite Juang RH (2004) BCbasics

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