Biotechnology: Status and Utilizations.

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PCR and Detection of Y and X DNA. Hy-Antigen. Octopus Springs - Yellowstone National Park ... CHROMOSOME. Human 2.9. Dairy cattle 3.8. Chinchilla 7.5. Turkey 0. X Chromosome ...
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Biotechnology: Status and Uses Animal Science 434 John J. Parrish

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Reproductive Biotechnology\'s Artificial insemination In vitro incipient organism generation In vivo fetus creation Embryo exchange Gender choice Stem Cells Genetic building Cloning

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In Vitro Production of Embryos Oocyte seclusion and development Sperm planning Sperm capacitation Fertilization Embryo Development

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In Vivo Embryo Production Normal cycling female stallions Superovulated female Cattle Sheep Goats Deer Humans

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PGF 2 an eCG or FSH Multiple Ovulations Estrus Progesterone From C.L. In the first place Follicular Wave 10-12 Stimulating Follicular Development

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Non-Surgical Embryo Flush Day 6 or 7

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Stage of Embryo at Recovery Tight Morula (day 5 - 7) Early Blastocyst (day 7 - 8) Blastocyst (day 7 - 9)

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ET - Scheme

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Embryo Sexing Hy Antigen Associated with male cells PCR and Detection of Y and X DNA

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Octopus Springs - Yellowstone National Park Home of Thermus aquaticus - Taq Polymerase

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PCR Approach to Embryo Sexing Y X F M F

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Sperm Gender Selection of X or Y sperm

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PERCENT DNA DIFFERENCES BETWEEN X and Y CHROMOSOME Human 2.9 Cattle 3.8 Chinchilla 7.5 Turkey 0 X Chromosome has more DNA!!!

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Flow Cytometer Separation of X and Y Sperm Stained With DNA Sensitive Fluorescent Dye 9 0° L A S E R - + - Y s o r t X s o r t

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Sperm Gender Selection Flow Cytometry Only technique that works! Not very many sperm recuperated Reduced ripeness Expense will restrict use

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Fetal Sexing OR Ultrasound Evaluation Day 55 - 65

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Cloning Split morula

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Cloning by Nuclear Transfer Cycles are constrained Only 3 - 4 cycles

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Nuclear Cloning to Produce Stem Cells of Interest Grown in Culture Dish Mature Oocyte enucleated and cell of interest combined Embyro?? Creates Potential Tissue: Neural Adipose Connective Heart Differentiate Inner Cell Mass Cells disengaged and developed in society

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Stem Cells Grown in Culture and Allowed to Differentiate

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Benefits of Stem Cell Research Regenerative pharmaceutical and tissue building In vitro frameworks: drug revelation, toxicology, symptomatic measures and cell society reagents Biomedical exploration: disentangle instruments of illness and human advancement

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Genetic Manipulation

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Transgenic for Growth Hormone

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Gene Transfer Using Micro-Injection of Pronuclei Less than 1% productivity

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Gene Transfer Using Viral Transfection Better achievement yet left with potential for viral replication

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