"CSI" Plant Style: From the Research center to the Wheat Field.


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“CSI” Plant Style: From the Laboratory to the Wheat Field. Top Grant . Find new, important, qualities and QTLs Deploy them into adjusted mixed bags around the nation Use marker-helped choice techniques to upgrade quality organization. Top Grant.
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"CSI" Plant Style: From the Laboratory to the Wheat Field

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CAP Grant Discover new, profitable, qualities and QTLs Deploy them into adjusted assortments around the nation Use marker-helped choice methodologies to improve quality organization

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CAP Grant 16 establishments around the nation coordinate reproducing endeavors 4 year stipend began in 2006 Once again 20 cultivars/germplasm as of now discharged (http://maswheat.ucdavis.edu)

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Impact of Wheat in Washington In 2006 Excess of 2.2 million sections of land planted 81% winter wheat, 19% spring wheat Over 140 million bushels gathered Net return of $615 million

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From Problem to Lab to Wheat Field

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What Traditional Problems Do We Face

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New Characteristics Needed Drought resilience Disease and Insect resistance/resilience Herbicide resistance/resistance High yield Excellent end-use quality (High GPC)

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DNA—Deoxyribo nucleic corrosive that conveys the hereditary data in a phone Gene—a succession of DNA that involves a particular area and decides a specific trademark Definitions

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Definitions Allele-one individual from a couple of qualities that possesses a particular area on DNA a= = homozygous predominant = homozygous latent = heterozygous

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Punett Square

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Crossing Plants are distinguished which have the attribute and which require the characteristic Cross is made to introgress attribute Survivor, no diversion X Rock-star yielder, bites the dust 10 Seeds (all ) 25 ( ) 50 ( ) 25 ( ) Self fertilization

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Crossing Cross is made to introgress characteristic Survivor, no amusement X Rock-star yielder, bites the dust 10 Seeds (all ) 25 ( ) 50 ( ) 25 ( ) Self fertilization Survivor, Rock-star yielder?

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How Do We Determine Which Seeds Carry The Trait We Are Interested In?

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So, which of you plants are impervious to contagious ailments?

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I am! I am! I am! I am! I am! I am!

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Definitions Molecular Markers—DNA portions that can be utilized as banners to track qualities Linkage—happens when specific qualities are acquired together Linked Markers—markers which are nearly connected with a quality

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DNA Gene Marker We know the DNA arrangement of the marker Sometimes we don\'t have the foggiest idea about the succession of the quality we are searching for We can utilize the marker to in a roundabout way select for the quality of interest

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Tightly Linked Marker Gene Marker Gene 95% sure Non-connected Marker Gene half sure

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Marker-Assisted Selection Identify marker which is connected to quality We can remove DNA from the plants and search for the nearness of the marker This will let us know which have the attribute and which don\'t

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Picture of gathering tissue

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1 2 3 4

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+ 95% Ethanol

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PCR Explanation Polymerase Chain Reaction Used to open up DNA by means of enzymatic replication Allows a little measure of DNA to be utilized for investigation PCR response http://www.danquinnart.com/wheatcap/index.htm

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Gel Electrophoresis Allows partition of PCR item DNA embedded into agarose gel DNA sections go with ebb and flow Smaller pieces will travel speedier than substantial pieces

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Gel Analysis

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Gel Analysis

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Gel Analysis

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Gel Analysis

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Gel Analysis

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So, which of you plants are impervious to contagious illnesses?

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Evaluation After MAS has recognized which plants convey the attribute, further assessment is done in the field to ensure the plants are gainful Time to discharge an assortment is abbreviated Why might this be essential?

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Zak + 1 quality

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Tara 2002 Trait Selection Crossing From Laboratory to Wheat Field Washington Farmers Shepherds Grain WSU Food Services

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Acknowledgments

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