Enterobacter sakazakii General Microbiology .

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Enterobacter sakazakii General Microbiology. Contaminants and Normal Toxicants Subcommittee Meeting Walk 18, 2003. Maria Nazarowec-White PhD Science Assessment Unit Canadian Sustenance Examination Organization. Diagram. History Biology Occurrence on Canadian retail showcase Development attributes
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Enterobacter sakazakii General Microbiology Contaminants and Natural Toxicants Subcommittee Meeting March 18, 2003 Maria Nazarowec-White PhD Science Evaluation Unit Canadian Food Inspection Agency

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OVERVIEW History Ecology Incidence on Canadian retail showcase Growth qualities Phenotype portrayal Genotyping Pathogenicity

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History Name Enterobacter sakazakii proposed in1980 DNA hybridization concentrates Biochemical responses Yellow - pigmented states

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Entrobacter sakazakii province

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Electron micrograph of Entrobactor sakazakii

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Surface water Mud Rotting wood Bird dung Rodents Domestic creatures Cow\'s drain E. sakazakii and the earth

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Clinical Strains of E. sakazakii London 2 Montreal 1 Toronto 3 LCDC 3

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Dried-newborn child recipe confines Company Positive examples (%) A 3/24 (12) B 2/24 (8) C 0/24 (0) D 1/24 (4) E 2/24 (8) total 8/120 (6.7)

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Temperature Gradient Incubator 10 strains Temperature run 4-50°C 30 tubes in copy

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Temperature Gradient Incubator C = Clinical F = Food

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Experimental Protocol Growth Study 3 Different formulae Cocktails 5 clinical detaches 5 sustenance disengages 4, 10 and 23°C Direct plating Generation and slack time

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Phenotyping Biochemical 3 biotypes Antibiograms 4 antibiograms

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Ampicillin Cefotaxime Chloramphenicol Gentimicin Kanamycin Polymixin B Streptomycin Trimethoprim-Sulfamthoxazole Tetracycline Sulfisoxazole Cephalothin Antibiotics utilized

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Antibiotic Resistance Profile Antibiotic Number of strains (18) Ampicillin 1 Tetracycline 2 Chloramphenicol 3 Cephalothin 17 Sulfisoxazole 18

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Genotyping Ribotype Pulsed Field Gel Electrophoresis (PFGE) Random Amplification of Polymorphic DNA (RAPD)

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Genotypic profiles of E. sakazakii

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Virulence Factors Suckling mouse measure Three cell culture lines Infectivity intraperitoneal oral test

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Enterotoxin Screening 4 strains positive for enterotoxin creation 3 clinical and 1 sustenance

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Morphological Responses of Cell Lines to Filtrates 1 clinical strain poisonous to 3 cell lines more articulated on Y-1 and Vero cells no impact of bubbling on Y-1 and CHO cells cytopathic impact diminished on Vero cells 2 clinical strains, no impact on 3 cell lines

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Infectivity examines i.p. infusions deadly at 10 8 CFU per mouse inside 3 days of dosing per oral highway 2 strains brought on death 1 clinical and 1 nourishment

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Summary Incidence in dried baby equation on the Canadian market observed to be 6.7% Minimum development temperature 5.5-8.0°C Generation time at room temperature 40 min Four of eighteen strains deliver enterotoxin Infectious measurements Heterogeneity among strains

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What do we do now??

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