Hereditary Markers for Jatropha Biodiversity Assessment and Reproducing.

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Hereditary Markers for Jatropha Biodiversity Assessment and Reproducing HONG Yan, Gathering of Plant Biotechnology Prologue to jatropha Hereditary markers, different methods Controlled fertilization and hereditary mapping Our discoveries with SSR and AFLP on jatropha hereditary differences
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Hereditary Markers for Jatropha Biodiversity Evaluation and Breeding HONG Yan, Group of Plant Biotechnology Introduction to jatropha Genetic markers, different procedures Controlled fertilization and hereditary mapping Our discoveries with SSR and AFLP on jatropha hereditary differences Methylation touchy assorted qualities Implication/dialog

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“It has not been truly domesticated…. will likewise oblige genuine measures of agronomic research” Nature article

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Breeding/taming will build profitability and enhance other agronomic characteristics Maize Wild plants versus trained ones

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Genotype and environment Morphology and agronomic attributes are variable for jatropha Between assortments, additionally inside of mixed bags Variable execution of kin plants under same development conditions demonstrates the significance of genotype A couple provenance trials* led in Africa discovered: Genotype-environment connection (GxE) An incredible scope of seed yield Various unrefined fat substance from 18.4-42.3% Trait particular relative commitment stays to be further explored *Heller 1996

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Genetic Markers A quality or DNA succession having a known area on a chromosome and connected with a specific quality or characteristic Advantages of hereditary markers: Profile of hereditary markers will characterize a genotype Uniform at all tissues at all periods of the life form permits early location formatively stable and not influenced by the earth profoundly particular example can be produced for every individual Utilities: For biodiversity study To pick right folks for cross fertilization Marker helped rearing To separate plant mixed bags Quality control of ranch

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Comparison of Different hereditary marker procedures *ISSR as a variation Gustavo Caetano-Anolles and P.M.Gresshoff 1997

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Genetic marker improvement and biodiversity investigations of jatropha Few reports with RAPD and ISSR strategies Potential issues of unwavering quality and reproducibility Generally low hereditary differences reported in neighborhood populaces examined No report on worldwide jatropha hereditary differing qualities Basha 2007; Xiang 2007

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Self fertilization: for innate lines and assessing heredity of qualities Cross fertilization: to create mixture seeds between distinctive mixed bags, additionally with other Jatropha species for half and half power and new attributes Controlled fertilization for reproducing in TLL

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Interspecies jatropha crossovers Objectives: Genetic mapping Possible better characteristics F1 crossover plants

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Genetic marker advancement in TLL >500 SSR loci were cloned >50 groundwork mixes of AFLP examination High throughput fluorescence marker examination with ABI 3730xl DNA analyzer Both systems are utilized together for development of hereditary guide of jatropha with BC populaces Examples of SSR markers Example of SSR allele recognition

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SSR/AFLP assessment of jatropha biodiversity Work in advancement to break down 192 specimens from Asia, South America and Africa Preliminary discoveries: SSR examination indicated almost no assorted qualities, most were homozygous EcoRI/MseI AFLP indicated next to no assorted qualities, as well (polymorphic loci <5%, mean heterozygosity < 1%) Monomorphic band example appeared for most SSR loci

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Low hereditary differences as identified by general AFLP examination

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Methylation delicate AFLP Methylation touchy AFLP investigation PZ14A SU6 MD42 MD44 E2/H3-HpaII - methylation delicate E2/H3-MspI - methylation unfeeling X m5 C G C G C m5 Cut No cut

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Segregation of methylation touchy alleles

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Epigenetic assorted qualities in jatropha Methylation touchy AFLP Much higher assorted qualities saw in methylation touchy AFLP examination (polymorphic loci >60%, mean heterozygosity >14%) Stable in different parts of a plant Heritable, takes after Mendelian isolation Most differences found inside of populaces Biological essentialness not known yet

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DNA methylation in plant Best described epigenetic alteration Implicated in may critical organic procedures Interference of DNA methylation in Arabidopsis by down manage MET1, a methylase quality Inheritance of methylation example

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Discussion Reasons for the absence of biodiversity so far Technical or intrinsic? In the event that low hereditary differing qualities affirmed, issues to address: Genetic premise for phenotype? Rearing system? Any criticalness part of epigenetic in jatropha? Proposals: A worldwide study of hereditary differences is imperative and basic International cooperation and check is vital

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Temasek Life Sciences Laboratory (TLL) A non-benefit exploration establishment possessed by Temasek holding, NUS and NTU With 30 examination bunches, we chip away at both fundamental and connected science One focus of greatness in the region of plant tissue society, hereditary alteration, plant science and improvement study Experience and component to team up and work with industry A group with around 20 researchers taking a shot at jatropha: Biodiversity and rearing Genetic markers and mapping Platform innovation advancement Phytochemistry Genomics Molecular and cell science thinks about Two test homesteads 1 Research Link, National University of Singapore, Singapore 117604 Tel: +65 6872 7000 Fax: +65 6872 7007

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Jatropha International Congress December 17 - 18, 2008, Singapore First Circular Themes of the congress 1. Hereditary assets 2. Rearing advancements and procedures 3. Hereditary qualities and Genomics 4. Biosynthesis of unsaturated fats and poisons 5. Tissue society, proliferation and change 6. Manor administration and nuisance control 7. Oil handling 8. By-item use 9. Speculation and plans of action 10. Government approaches and motivators A True Event for Biofuel Research and Business Organized by Temasek Life Sciences Laboratory

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