Justen Andrews Peter Cherbas dgrc.cgbdiana .

Uploaded on:
Drosophila Genomics Resource Center. Justen Andrews Peter Cherbas http://dgrc.cgb.indiana.edu/. Drosophila Genomics Resource Center. Clones/vectors/RNAi/2-hybrid collection ~300,000, distribution >3,000 Cell lines collection ~ 100, distribution >200 Microarrays
Slide 1

Drosophila Genomics Resource Center Justen Andrews Peter Cherbas http://dgrc.cgb.indiana.edu/

Slide 2

Drosophila Genomics Resource Center Clones/vectors/RNAi/2-half and half accumulation ~300,000, appropriation >3,000 Cell lines gathering ~ 100, circulation >200 Microarrays Amplcon transcriptome microarrays, disseminated ~500 Oligonucleotide transcriptome microarrays (INDAC) pending Distribuion of genome tiling microarrays (White Russell) pending Users Individual: add up to 1,500, dynamic 600 Laboratory: add up to 650, dynamic 500

Slide 3

Amplicon transcriptome clusters Comprehensive amplicon microarrays Probes PCR increased utilizing quality particular preliminaries Donated by Incyte Genomics and Brian Oliver, NIDDK 15,552 spots = 10,000 non-redundent r3.1 qualities Quality control: gels, recolor, standardized identification, hybridization Shipped prepared for hybridization Gene records, QC information and conventions accessible for download at site

Slide 4

Systematic utilitarian comment of the transcriptome: the master plan Where are all the translation units, what are all the join variations, in which cells would they say they are communicated? Where are every one of the cis-administrative successions, what are the relating trans-elements, what is the administrative system graph through space and time? The arrangement is going to incorporate a scope of coordinated test and computational methodologies (near grouping investigation, transcriptional profiling, chip-chip) crossing a scope of authoritative levels (focuses, consortia, singular labs).

Slide 5

Resolution will increment after some time determination: high low genome tiling way microarrays: quality particular transcript particular species particular creatures organs tissue: cell sorts hereditary foundation association: decentralized brought together

Slide 6

Issues Microarray assets will advance as our insight base increments oligonucleotide tests against ebb and flow quality models will be generally steady microarray development ought to be by expansion as opposed to by upset with the goal that information is back perfect, eg quality particular - > exon/graft particular INDAC oligonucleotide exhibits serve as a valuable beginning stage for future variants: exon particular, to be utilized universally will it be conceivable to build up a Drosophila dish animal varieties transcriptome microarray(s)?

Slide 7

Issues Isolation of cell sorts is a prompt specialized test Dissection, LCM, FACS New innovations Is an office proficient/consistent?

Slide 8

Issues Integration between deliberate unified and de-incorporated methodologies Overall proficiency will be augmented if all units utilize basic reagents with perfect information Data guidelines and information warehousing/mining ought to be facilitated Should be tended to at the arranging stage

Slide 9

Issues Resource dispersion Specific arrangement now required by NIH Should be tended to at the arranging stage

Slide 10

DONORS Incyte Genomics Brian Oliver NIDDK Illumina BDGP CuraGen Walter Gehring Mitsubishi Institute Pat O\'Farrell Others GRANT SUPPORT NCRR NIGMS DGRC Peter Cherbas Thom Kaufman Kris Klueg Lucy Cherbas Jennifer Steinbachs Chris Hemerich Sally Todd ADVISORY BOARD Ken Burtis Reed George Alex Lash Brian Oliver Susan Parkhurst J Tim Westwood Kevin White

View more...