Oligo-asth nospermie Pour IAC: au moins 106 spz mobiles .


88 views
Uploaded on:
Category: Sports / Games
Description
Hypofertilité masculine Yves J.R Ménézo, PhD, Dr Sci UNILABS, Paris Genève Marc Cohen, MD, Clinique Natecia , Lyon. Several environmental Causes Chemicals (domestic use), electromagnetic fields, food. Une baisse de la fertilité masculine?.
Transcripts
Slide 1

Hypofertilité manly Yves J.R Ménézo, PhD, Dr Sci UNILABS, Paris Genève Marc Cohen, MD, Clinique Natecia , Lyon

Slide 2

Several natural Causes Chemicals (household utilize), electromagnetic fields, nourishment

Slide 3

Une baisse de la fertilité manly?

Slide 4

Electromagnetic radiations: Wifi, Mobile telephones… . Mobilite et viabilite diminue significativement apres article aux ondes electro magnetiques Augmentation de 8-OH-dG, and DNA fracture biomarqueurs du push oxydatif De Iuliis GN , Newey RJ , King BV , Aitken RJ .2008 Menezo and Cohen 2010

Slide 5

Métaux lourds, stretch oxydatif et spermatogénèse Des taux élevés de cadmium et de plomb sont associés an un niveau de push oxydatif élevé et à des dommages associés dans la lignée germinale AR , Aydemir B , Onaran I , Alici B , Ozkara H , Gulyasar T , Akyolcu MC .2007 Le taux endogène de glutathion et de GST ( glutathione S-transferase) sont inversement corrélés à la teneur en métaux lourds dans le sérum et le liquide séminal

Slide 6

Drogues et medicaments Cannabis : THC et spermatogenese!!! Steroides anabolisants Regulateurs du cholesterol et de la pression optimistic antidepresseurs, cimetidine, phenitoine, Li+ Traitements contre la chute des cheveux!!! Minoxidil et Propecia: à deconseiller fortement si spermatogénèse defaillante

Slide 7

estrogens : estrogens (USA), elevage Elimination dans l\'eau : Pillule fix

Slide 8

Endocrine disruptors Agriculture (water and sustenance) : pesticides (lindane...) and herbicides Xeno-oestrogens : affect as right on time as fetal life, irreversible Testicular dis-beginning (contortion GO in guys, apoptose germinal cells, Testicular tumor. Small scale penis Menezo et Cohen 2009

Slide 9

Disrupteurs endocriniens DDT: lessens sperm check (estrogen like) Detergents: alkylphenol polyethoxylate Vinclozin (fungicide): estrogenic antiandrogenic L PE : Usage domestique: DHEP: Bis(2-ethylhexyl)phthalate) plastifiant Jouets pour 1 er age, cosmetiques Plastic: Nonyphenol, Bisphenol, effet Disrupteur associe aà une enlargement du push oxydatif

Slide 10

Conséquences: décroissance de la qualité du sperme? Fixation, numeration mobilité? Oui mais controverses Danemark: Carlsen 1992 France: Auger et al 1995 Italie: Bilotta et al 1999

Slide 11

Modification de la morphologie (Cytogramme) Augmentation du % de formes anormales* oui, agreement Benshushan 1997 Andolz 1999 * including ADN quality in sperm

Slide 12

Acrosome Genetic material compacted Chromatine (protamines + DNA) Mid-piece Mitochondria « engine » « Propeller»

Slide 13

FERTILIZATION Intact sperm Acrosome response (after the cell has bound to ZP). After AR the acrosomal top has vanished

Slide 14

MORPHOLOGICALLY NORMAL SPERMATOZOA A. Spermac recolor : B. Papanicolaou recolor : C. Diff-Quick stain :

Slide 15

HEAD ABNORMALITIES A. Macrocephalic . Adjusted head with different degrees of broadening. The acrosome amplifies more remote than typical (we guess that these cells are frequently diploid = spermatogenesis deformity) B. Microcephalic with round head and nonappearance of the acrosome (serious pink recoloring of the core attribuable to a hypercondensation of sperm chromatin. Spermac stains the atomic segment of the head pink, corresponding to the level of atomic buildup)

Slide 16

MIDPIECE ABNORMALITIES A. Pseudodroplet . Mitochondria (m) have slackened proximally and have clumped distally (bolt = proximally bared axoneme) B. Mitochondrial misfortune . The mitochondria have confined totally (bolt) t = tail

Slide 17

Oligo-asthénospermie Pour IAC: au moins 10 6 spz mobiles

Slide 18

Determination de la quantité de spermatozoïdes efficaces II Impact de la morphologie 1-Les irregularities du flagelles et de la pièce intermédiaire peu favorables à la remontée des spz: IAC à déconseiller, image picked pour Acrosome 2-Anomalies de la têtes qualité de l\'ADN Nécrospermie signe généralement une fracture élévée (pic de l\'iceberg)

Slide 19

MIDPIECE ABNORMALITIES A. Broken neck . Head still joined to the center of the midpiece by method for a little cytoplasmic stalk (bolt) B. Broken midpiece . Midpiece folden back on itself in the center (bolt). This deformity is portrayed by extreme axonemal disturbance including crack abd disorder of fibrils.

Slide 20

TAIL ABNORMALITIES A. Minor « Dag » imperfection . Straightforward collapsing back of the tail arround a distal cytoplasmic bead (dd) B. Dag deformity. More extreme frame with tail wound once arround the midpiece (bolts)

Slide 21

ELONGATED HEAD FORMS A. Gentle extension with little sidelong central vacuole on the left side (acrosome in place) B. Mellow extension . Marginally more lengthened cell with narrowing in the postacrosomal district (acrosome in place)

Slide 22

SECONDARY ACROSOMAL ALTERATIONS A. Finish loss of acrosomal substance B. Acrosomal layer harm appeared by the fluffy blueprint of the acrosome (bolts) with unpredictable loss of acrosomal substance. Dim recoloring material (likely thickened midpiece and not cytoplasm). This harm is ordinarily observed after cryopreservation.

Slide 23

PRIMARY ACROSOMAL ABNORMALITIES A. Acrosomal sore (areola acrosome) = huge apical sore inside the acrosome, with purge focus (bolt). The round range simply over the central district is most likely additionally a blister inside the acrosome. B. Acrosomal sore . Extreme focal tropical pimple with lighter focus (bolt).

Slide 24

Sperm DNA discontinuity: Definition ADN DNA fracture = harms in the twofold strand due to « agression ». Apoptosis,ROS, irregularities in topoisomerase movement, sperm antibodies Breaks

Slide 25

Collecting Lens Photomultiplier Tube Stained Cells Excitation Source (Laser Beam) Flow Cytometry

Slide 26

SCSA ® - Acridine Orange Stained DNA 100 Native DNA Stainability (green fluorescence) 0 100 Fragmented DNA (red fluorescence)

Slide 27

HDS 3% DFI: 68%,

Slide 28

Dernier étape avant necrospermie? DFI Coeff. de corrélation de Spearman: rho = 0,65 ��  p < 2,2 x 10 - 16 100 80 60 40 20 NECROSPERMIE 20 40 60 80 100 Menezo 2007 140 patients

Slide 29

Age and DFI Age et fracture: Notre encounter (Ménézo et al. 2005) P<0.0001: 83 patients Y= 0.098X + 34.4 Menezo 2008

Slide 30

Apoptose , arret du cycle cellulaire Reparation , Tolerance CDC25 TP 53 Menezo 2007 OK +++ Mutagenesis, carcinogenesis)

Slide 31

ICSI avec sperm totalement fragmente 8modele souris) Fernandes-gonzales et al. Biol Reprod 2008 Anatomopathological examination of creatures at 16 months of age demonstrated some extensive organs and an expansion in pathologies (33% of CD1 females delivered with DFS introduced some strong tumors: lungs and dermis of back or neck. 20% of the B6D2F1 created with DFS passed on amid the initial 5 months of age, 25% of the surviving creatures demonstrated untimely maturing side effects 70% of mice kicked the bucket sooner than control with various sort of tumors. Menezo 2008

Slide 32

Antioxidants and fruitfulness L\'ovocyte an une capacité limitée à réparer l\'ADN Cette capacité diminue avec l\'Age maternel Plusieurs centaines de milliers d\'opérations de DNA repair pendant les premières 24 heures Post fecondation Ménézo and Cohen 2011

Slide 33

Antioxidants and richness Tenter de diminuer la charge de réparation de l\'ovocyte Tout dépassement de la capacité de reparation entrainera arrêt de développement embryonnaire ou Mutation

Slide 34

Les Anti-oxydants habituellement utilisés. Basés sur le vieillissement The part of free radicals and cancer prevention agents in generation. Agarwal A , Gupta S , Sikka S 2006 Ménézo 2009 Selenium Vitamine C (A+E) SOD Le vitamines du groupe B?

Slide 35

Sperm DNA discontinuity Se, vit C , Vit A , VIT E medicines: Δ HDS, 57 patients Increase in HDS DFI HDS Decrease in HDS with treatment Menezo et al. 2007

Slide 36

DFI/HDS and cell reinforcement medications : 57 patients 32.4 DFI p<0.0004 HDS p<0.0009 26.2 21,5 17.5 After treament Before treatment

Slide 37

Acrosome Genetic material compacted Chromatine (protamines + DNA) Mid-piece Mitochondria « engine » « Propeller»

Slide 38

Selenium - Sodium selenite induit l\'apoptose standard era de RLO° by means of les mitochondries (Wong 2009) - "Mitochondries principale source de RLO dans le sperme " (Aitken 2007) - Jamais de déficience en selenium decrite dans la "Caucasian populace " - Une cooperation se produit entre certaines structures associees au selenium et les "Zinc finger proteines" impliquées dans la réparation de l\'ADN Se: anticarcinogénique à faible fixation mais compromet la stabilité génétique à de strong points focuses : il affecte la möthylation de l\'ADN (Hartwig et al. 2003, Davis et al. 2000) Ménézo 2009

Slide 39

Antioxydants et fertilité Nouvelles approches Menezo and Cohen 2010

Slide 40

Glutathion : Sulfur Oxigen Nitrogen Carbone SH S-S

Slide 41

Antioxydants : I Quercetine *Folliquelia, procrelia, condensyl, Alleviative impacts of quercetin and onion on male regenerative poisonous quality incited by diesel fumes particles. Izawa H et al. Biosci Biotechnol Biochem. 2008 Stimulating impacts of quercetin on sperm quality and regenerative organs in grown-up male rats. Taepongsorat L, AsianJ Androl. 2008 Quercetin shields spermatogonial cells from 2,4-d-incited oxidative harm in embryonic chickens. Mi Y.J Reprod Dev. 2007

Slide 42

Absorption des polyphenols:Ils stimulent la synthese endogene de Glutathion . Estimation of GSH in mouse gastrocnemius (muscle) 3-4wks admission P<0.01

Slide 43

Betalaïnes (Betanine and Indicaxanthine) Indicaxanthine Betanine: purple red

Slide 44

B

Recommended
View more...