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5\'. 3\'. . 5\' TCA 3\'. How does DNA serve as a hereditary data bearer?. Polynucleotide grouping can convey complex data. . n=2, 16 distinctive conceivable successions (4x4)n=3, 64 diverse conceivable groupings (4x4x4)n=4, 256 diverse conceivable arrangements (4x4x4x4)n=5, 1024 diverse conceivable arrangements (4x4x4x4x4)n=6, 4096 distinctive conceivable arrangements (4x4x4x4x4x4)
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Slide 1

Section 2 The Molecular Nature of Genes II How does DNA go about as a hereditary data bearer? Page 24-35

Slide 2

3\' 5\' 5\'- 3\' OH 3\' 5\'

Slide 3

5\' end 3\' end 5\' TCA 3\'

Slide 5

How does DNA serve as a hereditary data bearer?

Slide 6

Polynucleotide succession can convey complex data n=2, 16 diverse conceivable arrangements (4x4) n=3, 64 distinctive conceivable groupings (4x4x4) n=4, 256 distinctive conceivable arrangements (4x4x4x4) n=5, 1024 distinctive conceivable arrangements (4x4x4x4x4) n=6, 4096 diverse conceivable successions (4x4x4x4x4x4) … .. n=y, 4 y distinctive conceivable successions

Slide 7

Chargaff\'s Rules Erwin Chargaff saw in 1950 that the substance of purines was dependably generally equivalent to the substance of pyrimidines. Besides, the measures of adenine and thymine were dependably generally equivalent, similar to the measures of guanine and cytosine. A=T, C=G, yet what does this mean?

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X-beam basic data Rosaline Franklin, 1952 What does this intriguing example mean? The DNA is a basic, general structure with rehashed units

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Crick Watson

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The helix must have the same width

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3.4å

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From the X-beam diffraction design, Crick likewise derived that DNA ought to be a twofold helix with the phosphate bunches on the outside and the bases within. How do the helies fit together? How are the nitrogen bases masterminded?

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In a snapshot of part knowledge and part good fortune, Waston understood that adenine could match intimately with thymine, and that guanine could combine intimately with cytosine. Additionally, the A/T base pair was about the same width as a G/C pair. All the more critically, this "base matching" concurred with and clarified Chargaff\'s perceptions: A=T and G=C

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Hydrogen bond

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Francis Crick concurred with Waston\'s speculation. He additionally called attention to that as a result of certain bond edges and the vicinity of the base combines, the two helices needed to keep running in inverse bearings. The helices are antiparallel to each other.

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Watson and Crick presented a 900 word original copy on their DNA structure expectation to Nature. In their paper, they composed: " It has not got away from our notification that the particular base blending we have proposed instantly recommends a conceivable duplicating system for the hereditary material. " This essentially serves as a rule on how DNA can be reliably duplicated or replicated into the people to come.

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Francis Crick Maurice Wilkins Rosalind Franklin James Waston

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A B Z An Inclination of base pair from flat Residues per turn Form Pitch Å A 24.6 ~11 +19º B 33.2 ~10 -1.2º Z 45.6 12 - 9º

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Although G=C and A=T are valid for each living being, the measures of G+C substance differ from creature to life form

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Two DNA strands can be isolated basically by warming, a procedure called DNA denaturation or DNA liquefying. The temperature at which the DNA strands are half denatured is known as the liquefying temperature, or T m . T m of a DNA is to a great extent dictated by its G/C%. The measure of DNA strand partition can be measured by the absorbance of the DNA arrangement at 260 nm light.

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The way toward rejoining the isolated DNA strands is called toughening or renaturation Temperature - 25ºC beneath T m . DNA focus - the higher fixation, the better the toughening. Renaturation time - the more extended the time, the better the toughening.

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toughening The way toward tempering a DNA strand with an integral or about corresponding RNA strand or DNA strand from an alternate cause is called hybridization.

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DNAs are of different sizes and shapes

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