Research facility Creature Taking care of Procedure.

Uploaded on:
Category: Food / Beverages
Rabbit. Objective. To agree to the Animal Welfare Ordinance and ... Thusly, we would advance our insight in the field of research center creature ...
Slide 1

Lab Animal Handling Technique - Mouse - Rat - Rabbit

Slide 2

Objective To consent to the Animal Welfare Ordinance and abstain from misusing of creature in exploration To give essential ideas of creature taking care of procedure to new creature client While offering our idea and strategies to our creature client, we additionally support remarks from experienced creature clients. Thusly, we would advance our insight in the field of lab creature research on both sides and further advantage creature welfare and in addition the validity of exploration result in our college

Slide 3

Laboratory Animal Handling Technique - Rat A.Intraperitoneal infusion B.Blood gathering from tail vein C.Blood accumulation from cardiovascular cut D. Oral bolstering E. Sexing

Slide 4

Intraperitoneal Injection in Rat The most widely recognized technique for regulating medications to rats

Slide 5

Tools for intraperitoneal infusion in rodent 75% liquor cotton ball for surface sanitization medium size towel for limiting the rodent 25G 5/8" needle with 1cc syringe for infusion

Slide 6

Let the rodent unwinds on the highest point of the top.

Slide 7

Stretch the body of the rodent by pulling up it\'s tail and afterward cover the rodent with a towel by your left hand

Slide 8

Fold the skirts of towel under the rodent from all bearings

Slide 9

Grasp up the left hindlimb of the rodent to uncover the guts

Slide 10

The infusion site ought to be in the lower left quadrant of the mid-region in light of the fact that fundamental organs are missing from this territory

Slide 11

Only the tip of the needle ought to enter the stomach divider to avert infusion into the guts.

Slide 12

Collection of blood from tail vein in rodent General anesthesia required little sum: 0.1-1 ml

Slide 13

Tools for accumulation of blood from tail vein 75% liquor cotton ball for surface sanitization 27G1/2" needle with 1 ml syringe for blood withdrawal a vial for blood gathering

Slide 14

Optimal site of blood withdrawal is around the distal 33% of the tail since this a player in tail gives better representation of the veins

Slide 15

Disinfect the tail with 75% alcoholic cotton ball

Slide 16

When the needle infiltrates the epithelium of the tail, pull back the plugger a bit to make negative weight inside the syringe, then push the needle in the vein gradually until blood get into the dead space of the needle head

Slide 17

Pull back the plugger by the ring finger to pull back blood from the tail vein

Slide 18

Using a surgical tool to make a little twisted on the tail is likewise a possibility for gathering blood from tail vein

Slide 19

Blood can be gathered b utilizing a pipetteman after then

Slide 20

Collection of Blood from Cardiac cut in Rat General anesthesia required Large sum: up to 3% of body weight

Slide 21

Tools for gathering of blood from cardiovascular cut 75% liquor cotton ball for surface cleansing 24G needle with 10cc syringe for blood pull back 15 cc rotator tube for blood gathering

Slide 22

Disinfect the left thorax with 75% alcoholic cotton ball

Slide 23

Search for the purpose of most extreme heart beat

Slide 24

Insert the needle straight on the chose point and pull back blood by your left hand

Slide 25

Oral Feeding in Rat Feeding sum constrained to 1% of body weight

Slide 26

Tools for oral sustaining in rodent 16 G ball-tipped bolstering needle with syring Leather glove

Slide 27

Restrain the shoulders of the rodent by your thumb and forefinger, then backing the lower appendages with your right hand

Slide 28

Restrain the tail of the rodent in the middle of your ring finger and little finger

Slide 29

Let the rodent lying to your left side palm and present the ball-tipped encouraging needle from the pharynx into the throat when the rodent is in the demonstration of gulping

Slide 30

Sexing Rat-the separation between the butt-centric and genital holes is more noteworthy in the male (left) contrasted with the female (right)

View more...