Research facility Creature Taking care of Procedure.


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Rabbit. Objective. To agree to the Animal Welfare Ordinance and ... Thusly, we would advance our insight in the field of research center creature ...
Transcripts
Slide 1

Lab Animal Handling Technique - Mouse - Rat - Rabbit

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Objective To consent to the Animal Welfare Ordinance and abstain from misusing of creature in exploration To give essential ideas of creature taking care of procedure to new creature client While offering our idea and strategies to our creature client, we additionally support remarks from experienced creature clients. Thusly, we would advance our insight in the field of lab creature research on both sides and further advantage creature welfare and in addition the validity of exploration result in our college

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Laboratory Animal Handling Technique - Rat A.Intraperitoneal infusion B.Blood gathering from tail vein C.Blood accumulation from cardiovascular cut D. Oral bolstering E. Sexing

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Intraperitoneal Injection in Rat The most widely recognized technique for regulating medications to rats

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Tools for intraperitoneal infusion in rodent 75% liquor cotton ball for surface sanitization medium size towel for limiting the rodent 25G 5/8" needle with 1cc syringe for infusion

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Let the rodent unwinds on the highest point of the top.

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Stretch the body of the rodent by pulling up it\'s tail and afterward cover the rodent with a towel by your left hand

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Fold the skirts of towel under the rodent from all bearings

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Grasp up the left hindlimb of the rodent to uncover the guts

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The infusion site ought to be in the lower left quadrant of the mid-region in light of the fact that fundamental organs are missing from this territory

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Only the tip of the needle ought to enter the stomach divider to avert infusion into the guts.

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Collection of blood from tail vein in rodent General anesthesia required little sum: 0.1-1 ml

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Tools for accumulation of blood from tail vein 75% liquor cotton ball for surface sanitization 27G1/2" needle with 1 ml syringe for blood withdrawal a vial for blood gathering

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Optimal site of blood withdrawal is around the distal 33% of the tail since this a player in tail gives better representation of the veins

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Disinfect the tail with 75% alcoholic cotton ball

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When the needle infiltrates the epithelium of the tail, pull back the plugger a bit to make negative weight inside the syringe, then push the needle in the vein gradually until blood get into the dead space of the needle head

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Pull back the plugger by the ring finger to pull back blood from the tail vein

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Using a surgical tool to make a little twisted on the tail is likewise a possibility for gathering blood from tail vein

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Blood can be gathered b utilizing a pipetteman after then

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Collection of Blood from Cardiac cut in Rat General anesthesia required Large sum: up to 3% of body weight

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Tools for gathering of blood from cardiovascular cut 75% liquor cotton ball for surface cleansing 24G needle with 10cc syringe for blood pull back 15 cc rotator tube for blood gathering

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Disinfect the left thorax with 75% alcoholic cotton ball

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Search for the purpose of most extreme heart beat

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Insert the needle straight on the chose point and pull back blood by your left hand

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Oral Feeding in Rat Feeding sum constrained to 1% of body weight

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Tools for oral sustaining in rodent 16 G ball-tipped bolstering needle with syring Leather glove

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Restrain the shoulders of the rodent by your thumb and forefinger, then backing the lower appendages with your right hand

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Restrain the tail of the rodent in the middle of your ring finger and little finger

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Let the rodent lying to your left side palm and present the ball-tipped encouraging needle from the pharynx into the throat when the rodent is in the demonstration of gulping

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Sexing Rat-the separation between the butt-centric and genital holes is more noteworthy in the male (left) contrasted with the female (right)

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