Similarity TESTING .


29 views
Uploaded on:
Category: Medical / Health
Description
drmsaiem. Similarity TESTING. Out line Collection and Preparation of SamplesCollecting Patient SamplesDonor SamplesSelection of Appropriate Donor UnitsTesting of the contributor sampleTesting of the patient sampleCross-matchSummary of Pre-transfusion Testing . drmsaiem. Similarity TESTING.
Transcripts
Slide 1

Similarity TESTING Dr. MOHAMMED H SAIEM ALDAHR BLOOD BANK 3 RD MEDICAL TECHNOLOGY drmsaiem

Slide 2

COMPATIBILITY TESTING Out line Collection and Preparation of Samples Collecting Patient Samples Donor Samples Selection of Appropriate Donor Units Testing of the contributor test Testing of the patient specimen Cross-coordinate Summary of Pre-transfusion Testing drmsaiem

Slide 3

COMPATIBILITY TESTING As the information of fresh recruits assemble framework expanded, so did the look for more touchy pre-transfusion similarity testing strategies. Pioneer blood brokers blended the patient\'s serum and the contributor\'s red cells and watched for direct red platelets lysis, agglutination, or both. This got to be distinctly known as the real cross-coordinate test. The term similarity test and cross-match are a few circumstances utilized conversely, they ought to be obviously separated. drmsaiem

Slide 4

COMPATIBILITY TESTING The cross-coordinate turned out to be a piece of a progression of pre-transfusion test known as similarity testing . The similarity test incorporates an ABO and Rh gathering performed on the contributor and beneficiary specimens, screening of the giver\'s and patient\'s sera for surprising antibodies, and a cross-coordinate. The reason for pre-transfusion or similarity testing is to guarantee the most ideal aftereffects of a blood transfusion. The transfused red cells will have a satisfactory survival rate, and there will be no critical decimation of the beneficiary\'s own particular red cells. drmsaiem

Slide 5

COMPATIBILITY TESTING Collection and Preparation of Samples . Positive Patient Identification The real reason for transfusion related fatalities have been administrative blunders bringing about off base ABO gathering The most normal reason for administrative mistakes and in this manner transfusion mischances is misidentification of the patient required in the transfusion. Disarray in recognizable proof of the patient when the blood test was drawn a stirred up tests amid taking care of in the lab, and blunder in distinguishing proof of the patient when the transfusion was given. drmsaiem

Slide 6

COMPATIBILITY TESTING To keep gathering of tests from the wrong patient , the blood ask for frame must be utilized to affirm the patient\'s personality before phlebotomy is performed . The ask for shape must express the proposed beneficiary\'s full name, and one of a kind healing center ID number. Other data, for example, age and date of birth, address, sex, and name of asking for doctor can be utilized to check persistent character promote however is not required on the shape. drmsaiem

Slide 7

COMPATIBILITY TESTING The patient\'s wrist band recognizable proof should dependably be contrasted and the demand shape. In the event that the patient does not have a wristband or if the patient\'s character is obscure, some type of positive recognizable proof must be joined to the patient before gathering of tests. drmsaiem

Slide 8

COMPATIBILITY TESTING Collecting Patient Samples Hemolyzed tests can not be utilized for testing since hemolysis brought about by enactment of supplement Serum or plasma might be utilized for pre-transfusion testing. Most blood donation center technologist favor serum since plasma may bring about little fibrin clumps to shape which may hard to recognize from genuine agglutination. drmsaiem

Slide 9

COMPATIBILITY TESTING When an example is gotten in the lab, a blood donation center technologist must affirm that the data on the specimen and demand shape concur. All disparities must be settled before the example is acknowledged, and if any uncertainty exists, another specimen must be drawn. drmsaiem

Slide 10

COMPATIBILITY TESTING Donor Samples. Benefactor testing tests must be taken when the full contributor unit is drawn. Contingent upon the technique utilized for testing, thickened example, hostile to coagulated specimens, or both, are gotten. Contributor data and therapeutic history card, the pilot tests for preparing, and the accumulation sack should all be named with a similar extraordinary number code before beginning the phlebotomy, and the numbers must be confirmed again instantly in the wake of filling . drmsaiem

Slide 11

COMPATIBILITY TESTING Donor and beneficiary specimens must be put away for at least 7 days taking after transfusion. The specimens ought to be stoppered and refrigerated at 1-6°C, deliberately marked, and sufficient in volume with the goal that they can be re-assessed if the patient encounters an unfavorable reaction to the transfusion. drmsaiem

Slide 12

Compatibility Testing Protocols. Determination of Appropriate Donor Units. In all cases, blood and blood segments of the patient\'s own ABO and Rh gathering ought to be chosen for transfusion. Whenever blood and blood segments of the patient\'s sort are inaccessible or when some other reason blocks their utilization, units chose must do not have any Ag against which the patient has a critical Ab. drmsaiem

Slide 13

Selection of Appropriate Donor Units. At the point when transfusion of an ABO assemble not quite the same as the beneficiary must be given, stuffed red cells must be utilized as opposed to entire blood which contains plasma Abs that are contrary with the patient\'s red platelets. Gather O stuffed red platelets can be securely utilized for all patients, be that as it may, protection of a constrained supply of gathering O blood ought to manage its utilization for patients of other AB sorts just in exceptional conditions. drmsaiem

Slide 14

Selection of Appropriate Donor Units. On the off chance that ABO-particular blood is not accessible or is in under satisfactory supply, elective blood gatherings are picked as abridged in the accompanying table; Patient \'s BG Alternative BG given as stuffed cells O NONE A O B O AB A, B, O just a single of the three ought to be utilized for a given patient drmsaiem

Slide 15

Selection of Appropriate Donor Units. Rh-negative blood can be given to Rh-positive patients, be that as it may, great stock administration again ought to ration this constrained asset for use in Rh-neg beneficiaries. On the off chance that Rh-neg units is close lapse, the unit ought to be given as opposed to squandered. drmsaiem

Slide 16

Selection of Appropriate Donor Units. Rh-pos blood ought not be given to Rh-neg ladies of childbearing age. Transfusion of Rh-neg male patients and female patients past menopause with Rh-pos blood is satisfactory the length of no performed against D is obvious in the sera . drmsaiem

Slide 17

Compatibility Testing Protocols. Testing of the giver test. As per the Code of Federal Regulation (CFR) and the American Association of BB (AABB) benchmarks , ABO and Rh gathering ( including a test for feeble D) and tests expected to anticipate infection transmission must be performed on an example of blood taken at the season of accumulation of the unit of blood from the giver. A screening test for surprising antibodies to red platelet Ags is required by AABB norms on tests from givers uncovering a past filled with earlier transfusion or pregnancy. drmsaiem

Slide 18

Testing of the contributor test The transfusing office is required by AABB norms to affirm the ABO cell gathering on all units and Rh gathering on units marked Rh-neg. Tests for powerless (D u ) are not required to be accounted for. The transfusion office does not have to rehash some other testing technique. drmsaiem

Slide 19

Testing of the patient example. A record of all outcomes acquired in testing tolerant examples must be kept up. Distinguishing proof number ought to be alloted every time a patient is conceded for treatment. Any errors amongst past and current outcomes must be settled before transfusion is started. Another specimen ought to be gathered from the patient, if important to determine the issue. ABO and Rh gathering results ought to be incorporated into the document. Additionally, documentations concerning uncommon serologic responses and the character of startling Abs in the patient\'s serum ought to be incorporated. drmsaiem

Slide 20

Testing of the patient example. ABO and Rh gathering and Ab screening of the patient\'s serum can be performed ahead of time of or in the meantime as the cross-coordinate. In the event that the patient has had a transfusion or has been pregnant inside the most recent 3 months or if the history is inaccessible or unverifiable, the example must be acquired from the patient inside 3 days of planned transfusion. drmsaiem

Slide 21

Testing of the patient specimen. ABO Grouping. Assurance of the patient\'s right ABO gathering is the most basic pre-transfusion serologic test. On the off chance that the cell and serum gathering comes about don\'t concur, extra testing must be led to determine the error. In the event that the patient\'s ABO gather can\'t be agreeable decided and prompt transfusion is fundamental, aggregate O pressed red platelets ought to be utilized. drmsaiem

Slide 22

Testing of the patient specimen. Rh Grouping. Rh gathering is performed utilizing against D blood gathering serum. Tube or slide tests ought to be performed by produce\'s headings for the reagent, which might possibly incorporate the utilization of a reasonable diluents control. Control must be keep running in parallel with Rh gathering tests performed on patient\'s examples, to maintain a strategic distance from inaccurate assignment of Rh neg, understanding as Rh positive. drmsaiem

Slide 23

Testing of the patient example. Coordinate antiglobulin test (DAT) ought to be performed on the patient\'s red platelets to figure out if take-up of autoantibody, (alloantibodies, if the patient\'s has been as of late transfused) is in charge of the positive control result. drmsaiem

Slide 24

Testing of the patient specimen. On the off chance that the Rh gathering of the beneficiary can not be resolved and transfusion is basic, Rh negative blood ought to be given. The test for D u is superfluous when testing transfusion beneficiaries . People writing as Rh neg in direct testing ought to get Rh-neg blood and those writing as Rh pos in direct testing ought to get Rh pos blood. A

Recommended
View more...