Open Field Testing of Rodents - PowerPoint PPT Presentation

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Open Field Testing of Rodents

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  1. Open Field Testing of Rodents

  2. Anxiety – Gross Motor Behavior - Open Field Test Observe species-common (behavior: rearing sniffing, grooming) Observe thigmotaxic behavior (stay at walls) 48 cm Place animal in a corner square for a designated amount of time then observe the number of squares transversed, rearing, defecation etc.  Anxiolytics serve to increase exploratory behavior

  3. 5 min test • DVs – What type of data do we have???? Nominal, Scale, Ratio??? • Latency (in sec) • Squares Transversed (#) • Rearing (#) • Asst. Rearing (#) • Grooming (#) • Fecal Boli (#) • Urination (#) • Thigmotaxic behavior * Already Taken (no more than 13.5 units) * Already Taken (no more than 13.5 units)

  4. IVs??? • Environment Standard, Enriched • Gender Male, Female What is the Design Notation? 2X2

  5. In your Method you need: Subjects??? Who are they? (age, gender) Where did you get them? (vendor) How are they housed? What is their L/D cycle? Protocol Approval? Apparatus??? What equipment will you be using???? (Model#, City etc…) Open Field Specifics Any other equipment Procedure??? Take notes!!!!

  6. What are your hypotheses? Can have more than one… Rats are nocturnal… Male vs Female… Older Rats….