UW La Crosse's MycoPhyte Discovery Group: Fighting Infection in Patients with Cystic Fibrosis
The MycoPhyte Discovery Group at University of Wisconsin-La Crosse is an interdisciplinary initiative that combines chemistry, microbiology, and biology to collect, screen, isolate, synthesize, and screen myco-phyto crude extract to find active compounds that can fight infections in patients with cystic fibrosis. This group conducts relatively rapid and inexpensive initial bioassay and screen tests to isolate active compounds, purify, solve structure, search literature, and protect intellectual property.
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About UW La Crosse's MycoPhyte Discovery Group: Fighting Infection in Patients with Cystic Fibrosis
PowerPoint presentation about 'UW La Crosse's MycoPhyte Discovery Group: Fighting Infection in Patients with Cystic Fibrosis'. This presentation describes the topic on The MycoPhyte Discovery Group at University of Wisconsin-La Crosse is an interdisciplinary initiative that combines chemistry, microbiology, and biology to collect, screen, isolate, synthesize, and screen myco-phyto crude extract to find active compounds that can fight infections in patients with cystic fibrosis. This group conducts relatively rapid and inexpensive initial bioassay and screen tests to isolate active compounds, purify, solve structure, search literature, and protect intellectual property.. The key topics included in this slideshow are MycoPhyte Discovery Group, University of Wisconsin-La Crosse, cystic fibrosis, interdisciplinary, chemistry, microbiology, biology, active compounds, bioassay, intellectual property,. Download this presentation absolutely free.
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1. UW-L Chemistry Microbiology Biology University of WisconsinLa Crosse MYCOPHYTE DISCOVERY GROUP Fighting Infection in Patients with Cystic Fibrosis Bill Schwan UW-La Crosse
2. UW-L Chemistry Microbiology Biology MYCO-PHYTE DISCOVERY GROUP a UW-La Crosse interdisciplinary initiative Chemistry Microbiology Biology University of WisconsinLa Crosse
3. UW-L Chemistry Microbiology Biology University of WisconsinLa Crosse General procedure: collect screen isolate screen elucidate synthesize derivatize screen screen screen myco / phyto crude extract X inactivestop X inactivestop active partition & bioassay initial bioassay (other screens?) RELATIVELY RAPID (and inexpensive) MUCH SLOWER (and costly) more bio (and tox) screens synthesis analogs protect IP pre-clinical studies clinical trials MYCOPHYTE DISCOVERY GROUP activego inactivestop Isolate active cmpd(s) purify solve structure search literature protect IP
4. UW-L Chemistry Microbiology Biology MYCOPHYTE DISCOVERY the multidisciplinary teamthese people and more! Prof William Schwan Microbiology, UW-L Prof Tom Volk Biology, UW-L Prof Marc Rott Microbiology, UW-L Prof Aaron Monte Chemistry, UW-L The WiSys Team: Mindy, Bill Maliyakal, Bethany, Lisa Jonathan Fritz, JD, MS, BS Whyte, Hirschboeck, & Dudek, S.C. Dr. Joe Toce Chemistry, UW-L Prof James Cook & M. S. Kabir Chemistry, UW-M Dr. Xueting Liu Chemistry, UW-L University of WisconsinLa Crosse
5. UW-L Chemistry Microbiology Biology University of WisconsinLa Crosse progress / collaborative opportunities MYCOPHYTE DISCOVERY MD-1 Gm+, VRE, MRSA, TB solved: 2 active compounds no yes yes underway MD-2 Gm rods solved: 2-AQ no underway no underway MD-3 Gm+, potent VRE, MRSA partial solution: complex probably MD-4 TB solved: grifolin no MD-5 potent, broad antifungal, etc purification underway MD-6 broad, potent purification underway MD-7 N. sicca / gonorrhoeae purification underway PROJECT ACTIVITY STRUCTURE NOVEL? SYNTHESIS NOVEL ANALOGS PRE-CLINICAL CLINICAL + ~300 wild mushroom species with wide variety of activities + ongoing collaborations w/ WI Native Americans / herbal remedies COULD SHARE CRUDE EXTRACT LIBRARY TO SCREEN FOR ADDL ACTIVITIES AND HERE! NEED HELP HERE *
6. UW-L Chemistry Microbiology Biology University of WisconsinLa Crosse MYCOPHYTE DISCOVERY GROUP Screened fungal fruiting body library against bacteria that colonize the lungs of Cystic Fibrosis patients Goal: Find new antibiotic Project MD-2/F250
7. UW-L Chemistry Microbiology Biology University of WisconsinLa Crosse MYCOPHYTE DISCOVERY GROUP www.nlm.nih.gov What is Cystic Fibrosis?
8. UW-L Chemistry Microbiology Biology University of WisconsinLa Crosse MYCOPHYTE DISCOVERY GROUP What is Cystic Fibrosis? Homozygous recessive genetic disease afflicting Caucasian individuals Average lifespan of a CF patient is only 32 years Characterized by chronic bronchial infections that kill more than 90% of CF patients Antibiotic resistance in the bacteria a big problem
9. UW-L Chemistry Microbiology Biology University of WisconsinLa Crosse MYCOPHYTE DISCOVERY GROUP Screened a library of 260 fungal fruiting bodies Used disk diffusion assays for screening Target organisms were Pseudomonas aeruginosa and Acinetobacter baumannii Best hit was against fungus labeled F10/F250 Expanded the assay to include Burkholderia cepacia and Stenotrophomonas maltophilia Screening Protocol
10. UW-L Chemistry Microbiology Biology University of WisconsinLa Crosse MYCOPHYTE DISCOVERY GROUP Disk Diffusion Assays Against Glucose Nonfermenting Gram-negative Rods
11. UW-L Chemistry Microbiology Biology University of WisconsinLa Crosse MYCOPHYTE DISCOVERY GROUP Purification of the Active Drug First, thin-layer chromatography Second, HPLC separation of active peak Elucidation of structure of active drug using Mass Spectrometry NMR Spectroscopy
12. UW-L Chemistry Microbiology Biology University of WisconsinLa Crosse MYCOPHYTE DISCOVERY GROUP Structure of Active Natural Product Drug
13. UW-L Chemistry Microbiology Biology University of WisconsinLa Crosse MYCOPHYTE DISCOVERY GROUP 2-AQ MICs Against Glucose Nonfermenting Gram-negative Rods
14. UW-L Chemistry Microbiology Biology University of WisconsinLa Crosse MYCOPHYTE DISCOVERY GROUP What To Do Next? Synthesize 2-AQ Make analogs of 2-AQ Proceed with pre-clinical testing of 2-AQ More cytotoxicity testing in vitro Safety testing in mice/rats Efficacy testing in mice/rats
15. UW-L Chemistry Microbiology Biology University of WisconsinLa Crosse MYCOPHYTE DISCOVERY GROUP Possible 2-AQ Analogs
16. UW-L Chemistry Microbiology Biology University of WisconsinLa Crosse MYCOPHYTE DISCOVERY GROUP Synthetic Route #1 Merck Patent Approach
17. UW-L Chemistry Microbiology Biology University of WisconsinLa Crosse MYCOPHYTE DISCOVERY GROUP Synthetic Route #2 Wittig-Style Approach
18. UW-L Chemistry Microbiology Biology University of WisconsinLa Crosse MYCOPHYTE DISCOVERY GROUP Acknowledgements Mycophyte Discovery Co-Founders: Aaron Monte, Marc Rott, Joe Toce, Tom Volk UW-L Helpers: Craig Dunek, Kathleen Engelbrecht, Mike Gebhardt, Aaron McCoy, Ron McKelvey, Maria Drieta, Will Fiers, Nathan Connell Others: David Lewis, Jim Cook, Maliyakal John Monetary Assistance: UW-L, UW-System ARG and ARG-WiTAG grants
19. MYCOPHYTE DISCOVERY become a player in WI biotech? be the first startup in Western WI? can it be done? UW-L Chemistry Microbiology Biology University of WisconsinLa Crosse business opportunities?