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"KEMRI Polio Lab Report

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  1. “KEMRI Polio Lab Report 7th Inter-Country meeting of Directors of poliovirus laboratories in the WHO-EMRO,The Hashemite Republic Of Jordan August 24-26, 2003

  2. Kenya Medical Research Institute 24 years of Excellence in Health Research

  3. KEMRI Polio LAB. • Part of the Global Polio Lab. Network since 1995 • Designated National/Inter-Country Lab. Serving Kenya, southern Sudan, Somalia, Djibouti, Eritrea , the Seychelles and St. Hellena islands (THE HORN OF AFRICAN REGION). • Support KEPI in Vaccine Potency Testing For (Measles, YF, Polio) and field surveillance • Supported by KEMRI, WHO (HQs, AFRO & EMRO), JICA and CDC

  4. EPI Lab. Management Structure Lab. Director Measles Lab. Manager Polio Lab. Manager Lab. Technical Supervisor Measles Lab Tissue Culture Polio Lab. Technologists , Engineer & Technicians

  5. STAFF DISPOSITION • CURRENT ACTIVE STAFF [100%] IN POLIO LAB - One Research Officer ( Lab. Director) Dr. P. Borus • One Research Scientist (Manager & Safety Officer) New Staff • Two Lab. Technologists Mr. P. Kingori Mr. M.Nth’iwa -One Senior Technician Mr Muchiri -Two Junior Technicians Ms J. Hassan and Ms E. Njeri • An Institute Engineer Mr. Okondo • Two Laboratory Assistants Mr. B. Mwangi and Ms.J. Lewett

  6. Internal Quality Control • Cell Sensitivity testing • Receive our cells (RD & L20B) with low passages from NICD in South Africa Done Quarterly. • Received NIBSC Polio Virus Reference Sabin strains. • Proficiency Testing - WHO-AFRO 100% (July 2003) • External Assessment by CDC & WHO ( 2002) • Regular Monitoring of Techniques • Standard Operating Procedures (SOPs) 2003 and WHO Manual used

  7. Internal QC (3) • Equipment • Calibration of equipment done by Kenya Bureau of Standards • Regular maintenance/servicing of equipment done by the Institute Engineer

  8. Networking • Meetings with KEPI on a weekly basis to reconcile data and to discuss surveillance issues • Relationship with WHO (WR, AFRO ,EMRO& Hqs) good. WHO Provide us With the Lab.: • Supplies • Assistance on data management • Internet connection and air conditioner • Useful exchange of ideas during AFRO meetings , during accreditation or external assessment • East Africa bloc Bulletins

  9. Important Operational Practices • Monthly In-house meetings • In-House computer Training in place. • Weekly meetings with KEPI • Recruited an Institute Engineer

  10. LABORATORY CONTAINMENT OF WILD POLIO VIRUS • MANY TYPES OF CONTAINERS USED. • WE HAVE REQUESTED FOR STANDARD STOOL CONTAINERS FROM GENEVA. • STOOLS STORED IN ORIGINAL CONTAINERS. • WATER PROOF TAPE STRAPPED AROUND THE CAP OF THE CONTAINER. • ALL CONTAINERS PACKED IN ONE AUTOCLAVE BAG FOR EACH MONTH AND DISCARDED AFTER ONE YEAR.

  11. Results- 1999 Country Sample Case P1 P2 P3 Somalia 82 41 4S 0 2Wild Sudan 65 33 2S 0 2Wild Djibouti 2 1 0 0 0 Total 149 75 6S 0 4Wild KENYA 490 245 14S 0 1S

  12. Surveillance Indicators -1999

  13. Results 2000 Country sample Cases P1 P2 P3 Somalia 313 157 17S (73W) 26S 15S (6W) Sudan 116 58 4S 1S 0 Djibouti 4 2 0 0 0 TOTAL 433 217 21S (73W) 27S, 15S(6W) KENYA 609 304 24S 10S 29S

  14. Surveillance Indicators 2000 KENYA 53.5% 80.6% 99.2% 9.6%

  15. Results 2001 Country sample Cases P1 P2 P3 NPENT % Somalia 261 131 10 12 20(13W)36 13.8 Sudan 173 86 5(1W) 0 0 24 13,9 Djibouti 2 1 0 0 0 0 0 Eritrea 34 17 0 0 0 5 7 Total 470 235 15(1W)13 20 (13W) 65 11.7 KENYA 542 271 8 9 2 38 7.0

  16. SURVEILLANCE INDICATORS 2001

  17. Results 2002 Country sample Cases P1 P2 P3 NPENT % Somalia 216 108 9 18 14(6W) 23 10.6 Sudan 175 88 0 0 0 29 16.5 Eritrea 72 36 0 0 0 8 11.0 Total 463 232 9 18 14(6W) 60 12.9 KENYA 506 256 2 4 3 32 6.3

  18. SURVEILLANCE INDICATORS 2002

  19. RESULTS (AUGUST)- 2003

  20. SURVEILLANCE INDICATORS -2003 100 0

  21. SAMPLES HANDLED BY KEMRI LAB 2000-2003

  22. PLAN OF ACTION- 2003 • Strengthen regular contacts between the Lab. With KEPI. • To bring representatives from WHO-EMRO (Somalia , Djibouti, Sudan) counterparts on board during our weekly meetings (Present once a Month). • To identify contact persons in St. Hellena and Seychelles Islands. • Training of 2 staff in NICD South Africa .

  23. Recommendations • The number of countries supported and samples processed by the laboratory for EMRO has increased over the years- EMRO to consider more support to the lab in terms of : • Increasing the amount of money to USD 10,000 • ASSIST LAB. WITH TRANSPORT-VEHICLE. • Training (Cell Culture /attachment of staff in their labs). • Invitations to EMRO meetings encouraged.

  24. ACKNOWLEDGEMENTS -KEMRI,KEPI,JICA, WHO (HQs, AFRO & EMRO), CDC FOR SUPPORT