Biotechnology Explorer: Pop Culture Science for STEM Education

1. Pop Culture Science Bio-Rad Biotechnology Explorer IDEA and STEM Kits

2. Biotechnology Explorer | explorer.bio-rad.com 2 Curriculum and Training Specialists Leigh Brown leigh_brown@bio-rad.com Sherri Andrews, Ph.D. sherri_andrews@bio-rad.com Damon Tighe damon_tighe@bio-rad.com

3. Biotechnology Explorer | explorer.bio-rad.com 3 Bio-Rad Resources @BioRadEducation Biotechnology: A Lab Skills Course Pinterest.com/teachbiology bit.ly/b-rdropbox

4. Biotechnology Explorer | explorer.bio-rad.com 4 Workshop Overview What are we doing? Pop Culture + Lab Techniques How do we get students engaged? Movie scenarios Deeper dive into technique Choose your favorite movie scenario! Lab tailored to movie pick Standards alignment Other ideas? Results

5. Biotechnology Explorer | explorer.bio-rad.com 5 Getting Students Engaged

6. Biotechnology Explorer | explorer.bio-rad.com 6 A Few of your Students Favorite Movies?

7. Biotechnology Explorer | explorer.bio-rad.com 7 Electrophoresis separates molecules by CHARGE and SIZE Molecular sieve Electricity Electrophoresis: carry with water

8. Biotechnology Explorer | explorer.bio-rad.com 8 Lapse It! (10 second intervals)

9. Biotechnology Explorer | explorer.bio-rad.com 9 Interstellar Link to trailer of Interstellar movie: https://www.youtube.com/watch?v=2LqzF5WauAw

10. Biotechnology Explorer | explorer.bio-rad.com 10 Insterstellar https://www.youtube.com/watch ?v=zSWdZVtXT7E

11. Biotechnology Explorer | explorer.bio-rad.com 11 Interstellar Setting the scene : Crop blight has caused a massive die-off of most food crops. You are in a crew of astronauts who travel through a wormhole, searching for a new planetary home for humanity with a suitable food source.

12. Biotechnology Explorer | explorer.bio-rad.com 12 Interstellar Premise : You find some potentially edible specimens from an exoplanet near the wormhole, but some are poisonous, and contain a visible blue dye. Students must test the potential food source to find out if it contains the poisonous dye.

13. Biotechnology Explorer | explorer.bio-rad.com 13 Interstellar Teacher Prep: Blue 1 FD&C dye used for a standard (to represent poisonious blue dye) Medicine cup full of M&Ms or other candies (Nerds, Skittles) students can separate colors out into other cups. Lab Set Up : Each student has several specimens (M&Ms candy) to test. They use gel electrophoresis to determine if the blue dye is any of the specimens. poisonous blue dye potential food sources

14. Biotechnology Explorer | explorer.bio-rad.com 14 Divergent Link to trailer of Divergent movie: http://divergentthemovie.com/

15. Biotechnology Explorer | explorer.bio-rad.com 15 Divergent https://www.youtube.com/watch ?v=336qJITnDi0

16. Biotechnology Explorer | explorer.bio-rad.com 16 Divergent Setting the scene: You are a teenager in post- apocalyptic Chicago. Everyone is divided into 5 factions based on their aptitude and personality.

17. Biotechnology Explorer | explorer.bio-rad.com 17 Divergent Premise : During your 16 th year, you must decide whether to stay in your familys faction, or join a different faction and leave your family behind. There are several ways to determine which faction you belong in, including an aptitude test.

18. Biotechnology Explorer | explorer.bio-rad.com 18 Divergent After your aptitude test, results are now confirmed using a new technique to see what faction you most closely resemble. Do you fit in one faction? Or are you divergent, showing aptitude for 2 or more factions? **Results for Candor may be inconclusive. Hint: You might want to set out bowls of different colors of candies here for a visual for your students

19. Biotechnology Explorer | explorer.bio-rad.com 19 Divergent Teacher Prep: M&Ms or Skittles (5 different colors) to represent each faction FD&C dyes for student samples some should be a mix of 2 or more colors. Others should just be 1 color. Mix 1:1. Lab Set Up : Students will prepare extracts from the faction samples (candy). You might want to have these separated out into colors at the front of the room, each in its own bowl. These extracts will be run on a gel, along with 3 student samples. Students will identify their sample as matching one faction, or multiple ones (i.e. divergent), and decide if additional tests are needed or if the results are conclusive. student sample B faction samples student sample A student sample C

20. Biotechnology Explorer | explorer.bio-rad.com 20 Despicable Me 2 Link to trailer of Despicable Me 2 movie: https://www.youtube.com/watch?v=u6RxZK3LTnA

21. Biotechnology Explorer | explorer.bio-rad.com 21 Despicable Me 2 Setting the scene: PX-41 is a dangerous mutagen engineered by villians. It can turn minons into furry killing machines, capable of destroying everything in their paths. You are a reformed evil scientist, looking for an antidote to cure the mutated minions.

22. Biotechnology Explorer | explorer.bio-rad.com 22 Despicable Me 2 Premise: Using the materials available to you in your not- so-evil scientist laboratory, hatch a plan to purify different antidotes. You will need to: 1. Identify which samples contain the yellow antidote. 2. Are any of them pure? 3. Can you find another source for the antidote?

23. Biotechnology Explorer | explorer.bio-rad.com 23 Despicable Me 2 Teacher Prep: FD&C dye yellow, plus mixes of 2 or more other FD&C dyes (include yellow in a few) Optional M&Ms or Skittles for students to test for the presence of an antidote. Lab Set Up : Each workstation has a different set of potential antidotes, made from FD&C dyes. One is pure (yellow) the others are mixes of various dyes. Sample D Potential antidote sources (students pick 2 colors to test) Sample C Sample E Sample B Sample A pure antidote sample

24. Biotechnology Explorer | explorer.bio-rad.com 24 Before we starta little background What is electrophoresis?

25. Biotechnology Explorer | explorer.bio-rad.com 25 How can you separate molecules?

26. Biotechnology Explorer | explorer.bio-rad.com 26 Food and marker dyes have an intrinsic SIZE and CHARGE and thus can be separated using Electrophoresis Food dye Molecular weight Charge FD&C Blue 1 792.86 -2 FD&C Yellow 5 534.37 -3 FD&C Yellow 6 452.37 -2 FD&C Red 40 496.43 -2 Carminic Acid 492.38 0 Beetroot red 551.48 +1 Insert close up of FD&C dyes in kit

27. Biotechnology Explorer | explorer.bio-rad.com 27 DIY vs Commercial Electrophoresis Chamber

28. Biotechnology Explorer | explorer.bio-rad.com 28 Time to Pick! Interstellar Procedure - Interstellar Testing potential food source samples for poisonous blue dye Divergent Testing student samples to determine which faction(s) they belong to Despicable Me Testing samples to determine which ones contain the antidote, and if any are pure

29. Biotechnology Explorer | explorer.bio-rad.com 29 Procedure - Interstellar Before we begin: 1. Find the Blue 1 tube in your green rack. This will represent the poisonous blue dye that we are testing for. 2. Locate the potential food sources (M&Ms). 3. Find the Extraction Buffer tubes. 4. We wont be using any of the other tubes in the rack, so you can put them to the side. poisonous blue dye potential food sources Extraction buffer

30. Biotechnology Explorer | explorer.bio-rad.com 30 Procedure - Interstellar 1. (Already done for you). Place comb across box at 3cm mark. Pour molten agarose into box. Let solidify. Remove comb. Use a plastic ruler or card to cut across gel about 1 cm from each end, and scoop out and discard the rectangle of agarose. 2. Center gel in plastic chamber so that the wells are going across the box.

31. Biotechnology Explorer | explorer.bio-rad.com 31 Procedure - Interstellar 3. Add enough TAE buffer to completely cover gel. 4. Unfold paper clip electrodes as shown in diagram. Place one at each end of the gel. The bottom of the U will rest in the empty space at the top and bottom of the gel.

32. Biotechnology Explorer | explorer.bio-rad.com 32 Procedure - Interstellar 5. Separate M&Ms by color. Put one candy of each color into each cup. 6. Add 250 ul of extraction buffer to each cup. Swirl cups so dyes come off into the buffer. Extraction buffer 250 ul

33. Biotechnology Explorer | explorer.bio-rad.com 33 Procedure - Interstellar 7. Use the green 10 ul fixed volume pipet to load 10 ul of each of your samples into the wells. Change tips between each sample. Lane 1: Blue 1 (poisonous dye) Lane 2: Yellow M&M Lane 3: Red M&M Lane 4: Brown M&M Lane 5: Blue M&M Lane 6: Orange M&M Lane 7: Green M&M ***note: order is not important just be sure to write down what you did! Load 10 ul of each samplet

34. Biotechnology Explorer | explorer.bio-rad.com 34 Procedure - Interstellar 8. Assemble your battery tower as shown in the diagram. 9. Use the black alligator clip to clip your paper clip to the container at the end closest to your sample wells. Use the red alligator clip to attach the paper clip at the other end of the gel. Catching both the container and the paper clip with the alligator clip ensures that the paper clip wont fall over.

35. Biotechnology Explorer | explorer.bio-rad.com 35 Procedure - Interstellar 10. Start your gel by clipping the free end of the black alligator lead to the (-) terminal of your battery tower. Attach the free end of the red alligator lead to the (+) terminal of the battery tower. Look for bubbles. Let your gel run for about 15-20 minutes. 11. Compare the Blue #1 standard to your food samples, and see if any contain the poisonous blue dye.

36. Biotechnology Explorer | explorer.bio-rad.com 36 Procedure - Divergent Before we begin 1. Remove any green M&Ms from your candy cup we wont need them during the lab. The other colors will represent each faction. 2. Pick one sample (A, B, C, D, or E) for each person at your workstation. 3. Find the Extraction Buffer tubes. 4. We wont be using any of the other tubes in the rack, so you can put them to the side. student sample B faction samples student sample A student sample C Extraction buffer

37. Biotechnology Explorer | explorer.bio-rad.com 37 Procedure - Divergent 1. (Already done for you). Place comb across box at 3cm mark. Pour molten agarose into box. Let solidify. Remove comb. Use a plastic ruler or card to cut across gel about 1 cm from each end, and scoop out and discard the rectangle of agarose. 2. Center gel in plastic chamber so that the wells are going across the box.

38. Biotechnology Explorer | explorer.bio-rad.com 38 Procedure - Divergent 3. Add enough TAE buffer to completely cover gel. 4. Unfold paper clip electrodes as shown in diagram. Place one at each end of the gel. The bottom of the U will rest in the empty space at the top and bottom of the gel.

39. Biotechnology Explorer | explorer.bio-rad.com 39 Procedure - Divergent 5. You have representative samples from each faction in front of you. Separate these by color, putting one of each color into each cup. 6. Add 250 ul of extraction buffer to each cup. Swirl cups so dyes come off into the buffer. Extraction buffer 250 ul

40. Biotechnology Explorer | explorer.bio-rad.com 40 Procedure - Divergent 7. Use the green 10 ul fixed volume pipet to load 10 ul of each of your samples into the wells. Change tips between each sample. Lane 1: Yellow (Abnegation) Lane 2: Red (Amity) Lane 3: Brown (Candor) Lane 4: Blue (Erudite) Lane 5: Orange (Dauntless) Lane 6: Student A sample Lane 7: Student B sample Lane 8: Student C sample Load 10 ul of each samplet

41. Biotechnology Explorer | explorer.bio-rad.com 41 Procedure - Divergent 8. Assemble your battery tower as shown in the diagram. 9. Use the black alligator clip to clip your paper clip to the container at the end closest to your sample wells. Use the red alligator clip to attach the paper clip at the other end of the gel. Catching both the container and the paper clip with the alligator clip ensures that the paper clip wont fall over.

42. Biotechnology Explorer | explorer.bio-rad.com 42 Procedure - Divergent 10. Start your gel by clipping the free end of the black alligator lead to the (-) terminal of your battery tower. Attach the free end of the red alligator lead to the (+) terminal of the battery tower. Look for bubbles. Let your gel run for about 15-20 minutes. 11. Compare the student samples to the faction samples. Which faction do you belong to? Are you divergent?

43. Biotechnology Explorer | explorer.bio-rad.com 43 Procedure Despicable Me 1. Find the following tubes in your green rack: Yellow 5 (pure antidote sample) Samples A, B, C, D, and E from potential antidotes. Extraction Buffer 2. We wont be using any of the other tubes in the rack, so you can put them to the side. 3. Decide which 2 colors of candies might be a potential source of the yellow antidote. Take one of each of those 2 colors out, and put the rest of the candies aside. Sample D Potential antidote sources (pick 2 colors to test) Sample C Sample E Sample B Sample A pure antidote sample Before we begin Extraction buffer

44. Biotechnology Explorer | explorer.bio-rad.com 44 Procedure Despicable Me 1. (Already done for you). Place comb across box at 3cm mark. Pour molten agarose into box. Let solidify. Remove comb. Use a plastic ruler or card to cut across gel about 1 cm from each end, and scoop out and discard the rectangle of agarose. 2. Center gel in plastic chamber so that the wells are going across the box.

45. Biotechnology Explorer | explorer.bio-rad.com 45 Procedure Despicable Me 3. Add enough TAE buffer to completely cover gel. 4. Unfold paper clip electrodes as shown in diagram. Place one at each end of the gel. The bottom of the U will rest in the empty space at the top and bottom of the gel.

46. Biotechnology Explorer | explorer.bio-rad.com 46 Procedure Despicable Me 5. You have samples which potentially contain the antidote sitting in front of you. You also have some purified antidote. If youd like, you may test 2 M&M samples to see if they contain the antidote as well. 6. Add 250 ul of extraction buffer to each cup. Swirl cups so dyes come off into the buffer. Extraction buffer 250 ul

47. Biotechnology Explorer | explorer.bio-rad.com 47 Procedure Despicable Me 7. Use the green 10 ul fixed volume pipet to load 10 ul of each of your samples into the wells. Change tips between each sample. Lane 1: Yellow 5 (Antidote) Lane 2: A Lane 3: B Lane 4: C Lane 5: D Lane 6: E Lane 7: Yellow M&M Lane 8: Green M&M Load 10 ul of each samplet

48. Biotechnology Explorer | explorer.bio-rad.com 48 Procedure Despicable Me 8. Assemble your battery tower as shown in the diagram. 9. Use the black alligator clip to clip your paper clip to the container at the end closest to your sample wells. Use the red alligator clip to attach the paper clip at the other end of the gel. Catching both the container and the paper clip with the alligator clip ensures that the paper clip wont fall over.

49. Biotechnology Explorer | explorer.bio-rad.com 49 Procedure Despicable Me 10. Start your gel by clipping the free end of the black alligator lead to the (-) terminal of your battery tower. Attach the free end of the red alligator lead to the (+) terminal of the battery tower. Look for bubbles. Let your gel run for about 15-20 minutes. 11. Compare the purified antidote sample in lane 1 to the potential antidote samples. Which ones contain the antidote? Are any pure?

50. Biotechnology Explorer | explorer.bio-rad.com 50 What Can You Teach?

51. Biotechnology Explorer | explorer.bio-rad.com 51 Other Ideas?

52. Biotechnology Explorer | explorer.bio-rad.com 52 Results - Interstellar Blue # 1 Yellow M&M Red M&M Brown M&M Blue M&M Orange M&M Green M&M

53. Biotechnology Explorer | explorer.bio-rad.com 53 Results - Divergent Yellow (Abnegation) Red (Amity) Brown (Candor) Blue (Erudite) Orange (Dauntless) Student E Student B Student C Make new gel with Student A (yellow 6), B, E

54. Biotechnology Explorer | explorer.bio-rad.com 54 Results Despicable Me Antidote (Yellow 5) Sample A Sample B Sample C Sample D Sample E Yellow M&M Green M&M

55. Biotechnology Explorer | explorer.bio-rad.com 55 Teacher Prep and Lab Setup for all 3 scenarios 1. Create the following samples to be used in Despicable Me or Divergent. Mixtures should be equal parts. A: Yellow 6 B: Yellow 5 + Yellow 6 C: Yellow 5 + Blue 1 + Red 40 D: Blue 1 + Yellow 6 E: Yellow 5 + Blue 1 2. You will also need the following FD&C dyes: For Interstellar: Blue 1 For Despicable Me: Yellow 5 3. Each station also should have: 6 medicine cups 1 cup with M&Ms (mixed colors) Micropipet and tips Dye extraction buffer (2 ml) Gel STEM gel box (box, paper clip, alligator clips, batteries) Sharpie

56. Biotechnology Explorer | explorer.bio-rad.com 56 IDEA STEM Electrophoresis Kit IDEA/STEM Electrophoresis Kits Found in the 2014/2015 Catalog on pg. 48-49